Cytokine. 2006
Sep;35(5-6):253-7. Epub 2006 Nov 1.
Splenectomy changes the pattern of
cytokine production in beta-thalassemic patients.
Physiology
Research center of Kerman University of Medical Sciences, Kerman, Iran
.
.
Abstract
A major cause of morbidity and
mortality in beta-thalassemic patients is infections, assumed to be the result
of immunological changes. To determine the possible defect, we investigated the
cytokine productions by blood cells of beta-thalassemic patients using in-vivo
and in-vitro methods. Heparinized blood samples collected aseptically from 22
beta-thalassemic children aged 10-12yrs (half of them were splenectomized).
Samples from 10 healthy children served as control group. Part of samples was
used for evaluation of plasma IL-2, IL-10 and TGF-beta1. Other part were
stimulated with a mixture of LPS and PHA (1 and 10 microg/ml final
concentration), for different time period (4, 24, 48 and 72h). Results showed
circulating TGF-beta1 of splenectomized patients was significantly higher
(p<0.01) than the control group. In-vitro results showed IL-2 production of
patients' groups were significantly (p<0.01) lower than corresponding value
obtained for the control group. In addition, IL-10 production by splenectomized
group were less than other two group (p<0.01), while their TGF-beta1 were
higher (p<0.001) at all time points treated. In conclusion,
multi-transfusions could be responsible for a change in the subset of
circulating lymphocytes that could contribute to a state of partial immune
deficiency in beta-thalassemic patients, which is more prominence among the
splenectomized patient
Dr.Nada Fathy.
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